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KMID : 0191119900050010025
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Journal of Korean Medical Science
1990 Volume.5 No. 1 p.25 ~ p.31
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Antibody response of mouse splenocytes using mixture of supernatants of thymocytes, adherentand non-adherent splenocytes: in-vitro immunization-II
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Kim D
Noh GW/Kim DH/Kwon OH
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Abstract
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We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported several methods of in-vitro immunization using different conditioned media. Here we introduce another method of in-vitro immunization using a mixture of three types of supernatant (thymocytes, and adherent and non-adherent splenocytes of mouse). Splenocytes were immunized in-vitro by a recombinant human growth hormone (rhGH) with the above conditioned media, and the results by ELISA showed a much higher optical density than the other in-vitro immunization methods that we had previously reported. Humoral responses as a result of in-vitro immunization to soluble antigens were easily confirmed by ELISA using the above-conditioned media. This finding indicates that any other conditions thought to be critical by other researches may not be essential for in-vitro immunization.
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